In this paper , we introduce you the principle of time - resolved fluorescence immunoassay ( trfia ) study its test technique , analyze the structure of the instrument and introduce its application in biology and medicine 摘要本文介紹了時(shí)間分辨熒光免疫分析法的檢測原理、檢測方法,分析了時(shí)間分辨熒光免疫分析儀的結(jié)構(gòu)并介紹了其在臨床檢測方面的應(yīng)用。
2 . to establish a neutrajizing - inhabition time - resolved fluoroimmunoassay ( trfia ) of hepatitis b virus e antibody ( hbeab ) and hepatitis b virus core antibody ( hbcab ) based on the competion . the assay ranges of standard cures were 0 - 16ncu / mland 0 - 8ncu / ml . the within - run coefficient variations for standard samples were less than 10 % . compared this method with radioimmunoassay , the sensitivities were 92 . 8 % and 93 . 3 % 以中和抑制法建立了乙型肝炎病毒e抗體時(shí)間分辨熒光免疫分析法( hbeab - trfia )和乙型肝炎病毒核心抗體時(shí)間分辨熒光免疫分析法( hbcab - trfia ) ,標(biāo)準(zhǔn)曲線分析范圍分別為0 - 16ncu ml和0 - 8ncu ml 。
In conclusion , the diagnostic methods we developed for immunological markers of hepatitis b virus by using time - resolved fluoroimmunoassay , eu - dtta as label , have wide assay ranges , high sensitivity and specificity . they would have widely application in the further research work and clinical diagnosis 總之,我們利用eu - dtta為標(biāo)記物建立的乙型肝炎病毒五項(xiàng)血清學(xué)標(biāo)志物時(shí)間分辨熒光免疫分析法分析范圍寬,靈敏度高,操作簡便,具有優(yōu)越的定量檢測能力,十分適合臨床推廣應(yīng)用。