Construction of recombinant lactococcus lactis expressing porcine transmissible gastroenteritis spike glycoprotein and analysis of immunogenicity 基因的重組乳酸乳球菌的構(gòu)建及免疫原性分析
Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production 以lactococcuslactisnz9800為受體菌,研究了表達(dá)載體的表達(dá)情況。
In this paper , functional kefir yogurt starter was produced by lactococcus and lactobacillus isolated from kefir grains 摘要本文利用開菲爾粒中分離的乳球菌和乳桿菌采用混合發(fā)酵法研制開菲爾酸奶復(fù)合發(fā)酵劑。
Using a mouse model , the uk team has also found that a modified bacterium lactococcus lactis can be used to vaccinate against harmful infections 利用小鼠模型,該英國研究小組已經(jīng)發(fā)現(xiàn)了一種基因修飾細(xì)菌(乳酸乳球菌)可用于對有害傳染病的免疫接種。
4 . the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800 . sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production 抽提ecolijm109中的重組質(zhì)粒,轉(zhuǎn)化l . lactisnz9800 ,在含紅霉素的gm17平板上篩選轉(zhuǎn)化子。
This has resulted in the availability of genetically modified l . lactis with prospects for application in food industry . in this studay , genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper 乳酸乳球菌是一類長期應(yīng)用于食品發(fā)酵業(yè)的有益微生物,以乳酸菌為受體的表達(dá)系統(tǒng)具有安全、表達(dá)產(chǎn)物便于分離,使用方便等優(yōu)點(diǎn)。
A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid . the recombinant plasmid was transformd into e . colijm 109 and identified by restriction endonucleases analyzing . the recombinant plasmid was introduced into lactococcus lactis nz9800 本實(shí)驗提取乳酸乳球菌nizor5的基因組dna為模板,用pcr方法得到乳鏈菌肽前體基因( nisa ) ,克隆到乳酸乳球菌表達(dá)載體pmg36e ,構(gòu)建了表達(dá)載體pmg36e nisa 。
Compared with lactococcus , lactobacillus is the better choice of oral vaccine for some properties , including their immunogenicities , resistance to the circumstance of the alimentary tract and remaining in long period in gastro - intestinal tract , however , lactococcus is more effective in selecting antigen to induce immunological response , while the positive result of lactobacillus is limited 與乳酸乳球菌相比,乳酸桿菌特有的一些性質(zhì)使其成為口服疫苗更好的選擇,這些特性包括它們的免疫原性,對上消化道內(nèi)情況的耐受以及能在胃腸道中長時間留存,然而乳球菌在選擇抗原誘導(dǎo)免疫反應(yīng)方面更加有效,但是乳桿菌的陽性結(jié)果十分有限。
A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome . the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 " . conditions used for the pcr consisted of 30 cycles of 94 ? for0 . 5min , 45 ? for imin and 72 ? for 1 . 5min , plus one additional cycle of 72 for 10min 根據(jù)已發(fā)表的乳鏈菌肽前體基因的dna序列,設(shè)計兩條引物并引入酶切位點(diǎn)。以lactococcuslactisr5基因組dna為模板, pcr反應(yīng)條件: 94變性30s , 45退火60s , 72延伸90s ,反應(yīng)進(jìn)行30個循環(huán)。成功擴(kuò)出一條約460bp的dna片段。
百科解釋
Lactococcus is a genus of lactic acid bacteria that were formerly included in the genus Streptococcus Group N1. They are known as homofermentors meaning that they produce a single product, lactic acid in this case, as the major or only product of glucose fermentation.