Adequate quantities of plasma continued to perfuse the kidney . 相當(dāng)量的血漿持續(xù)灌注到腎臟。
The heart was perfused with a glutin solution of 30 % concentration . after that it was embedded with two steps to guarantee its anatomical position 以30 %濃度的明膠溶液灌注心臟,分兩步進(jìn)行包埋,以保證心臟處于解剖位。
Amiodarone prevent electrophysiological changes of acute atrial dilation accompanied with rapid atrial stimulation in isolated langendorff - perfused rabbit heart 離體兔心臟急性雙心房擴(kuò)張合并快速心房刺激時(shí)電生理變化及胺碘酮的干預(yù)作用
Tacho y , pappus t , lauffenburger m , et al . cgrp stimulates somatostat in release from isolated perfused rat stomach j . endocrinology , 1986 , 118 : 2144 雷銀雪,李兆申,湛先保,等.降鈣素基因相關(guān)肽在大鼠應(yīng)激性潰瘍發(fā)生中的作用j .第二軍醫(yī)大學(xué)學(xué)報(bào), 1998 , 19 ( 5 ) : 464 - 466
The isolated hepatocytes , isolated perfused organs , tissue slices , liver cell lines , subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays , especially the isolated hepatocytes 目前,常用的肝臟體外模型包括原代肝細(xì)模型、離體肝臟模型、肝臟切片模型、肝細(xì)胞系模型、亞細(xì)模型及基因工程細(xì)模型等,其中原代肝細(xì)型最?常用。
D ) pain and electroacupuncture stimulated group : after the injection of 200ul formaldehyde ( 5 % ) in the right hind paw , the rats are stimulated with electroacupuncture immediately ; the four groups of rats were perfused . sections were cut at 40um for the method of nadph - d 疼痛刺激加電針刺激組,在大鼠右側(cè)后腳掌注射5甲醛200ill后立即固定,并用針灸治療儀刺激同側(cè)后肢“足三里”穴3v5shz ,持續(xù)0
It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ) . in the present study a ventricular pressure - clamping system has been developed , which can be applied to isolated - perfused rabbit hearts . controlled by a computer , this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition , but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions , including perfusing , pacing , recording of electrocardiogram and monophasic action potentials , and clamping and measuring of ventricular pressures in isolated - perfused hearts . thus , it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level 在心臟機(jī)械電反饋的研究中準(zhǔn)確控制機(jī)械刺激是非常重要的.本研究室構(gòu)建了一套適用于離體家兔心臟的心室壓力鉗系統(tǒng).該系統(tǒng)通過計(jì)算機(jī)控制壓力鉗,不僅能模擬正常生理?xiàng)l件下左心室的壓力波形,還能在心室活動(dòng)周期的特定時(shí)相、以適當(dāng)波形對(duì)心室施加機(jī)械刺激.該系統(tǒng)集心臟灌流與起搏、表面心電圖記錄、單相動(dòng)作電位記錄、心室壓力鉗制與測(cè)定等多種功能于一體,特別適用于器官水平上觀察機(jī)械電反饋現(xiàn)象并探討其機(jī)制
Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ) . in the present study a ventricular pressure - clamping system has been developed , which can be applied to isolated - perfused rabbit hearts . controlled by a computer , this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition , but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions , including perfusing , pacing , recording of electrocardiogram and monophasic action potentials , and clamping and measuring of ventricular pressures in isolated - perfused hearts . thus , it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level 文摘:在心臟機(jī)械電反饋的研究中準(zhǔn)確控制機(jī)械刺激是非常重要的.本研究室構(gòu)建了一套適用于離體家兔心臟的心室壓力鉗系統(tǒng).該系統(tǒng)通過計(jì)算機(jī)控制壓力鉗,不僅能模擬正常生理?xiàng)l件下左心室的壓力波形,還能在心室活動(dòng)周期的特定時(shí)相、以適當(dāng)波形對(duì)心室施加機(jī)械刺激.該系統(tǒng)集心臟灌流與起搏、表面心電圖記錄、單相動(dòng)作電位記錄、心室壓力鉗制與測(cè)定等多種功能于一體,特別適用于器官水平上觀察機(jī)械電反饋現(xiàn)象并探討其機(jī)制
Half animals were perfused with paraformaldehyte following normal serum after test innnediatly , then removed the brain and fixed for 24 hours with paraformaldehyte , paraffin sections were prepared at 3um , histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals 結(jié)果如下: 1增殖細(xì)胞的觀察經(jīng)72h的快眼動(dòng)睡眠剝奪,實(shí)驗(yàn)組大鼠海馬齒狀回區(qū)brdu與p皿a ( proliferatingcellneuclearantigen , pma )陽(yáng)性標(biāo)記細(xì)胞數(shù)顯著增加。