The results suggested that human hscs can engraft into rat and differentiate into multilineage under certain microenvironment . thus , the human / rat xenograft model may provide a useful and convenient method for assay human hscs in vivo 新生大鼠免疫系統(tǒng)發(fā)育不完善,對異種抗原可形成免疫耐受,是很好的研究異種移植的實驗動物,且經(jīng)濟。
On the consideration of these problems and the fact that the rat immune system is not fully developed until birth , we tried to transplant neonatal rat with human hscs to test the possibility of this xenograft model 但此實驗模型操作復雜且實驗周期長,大多數(shù)實驗室不具備利用此實驗模型的條件。有研究證明,新生大鼠免疫系統(tǒng)發(fā)育不完善,對異種抗原可形成免疫耐受。
The acellular matrix treated by sds or glutaraldehyde should be cleaned by normal saline , or there will be cytotoxicity in it . the results of cell culture and implantation experiment demonstrate the high degree of cytocompatibibity and biocompatibility of this kind of acelluler matrix A半乳糖殘基是豬到人的主要異種抗原,會導致超急性免疫排斥,要實現(xiàn)豬到人的異種移植首先要去除的就是o gal 。
In one word , the methods for producing acellulace matrix have not been standardized . in this study , we want to select the best way to produce acellular matrix . we also use immunohistochemical method for detecting the magor heterogenetic antigen a - galactosyl residues ( a - gal ) in acellular matrix 并應用免疫組化的方法檢測脫細胞后基質(zhì)的主要異種抗原,用種植實驗觀察異種機體對脫細胞生物基質(zhì)的組織反應倩況,另在其上種植鼠成纖維細胞以觀察脫細胞生物基質(zhì)的細胞毒性和細胞相容問題。