Gfral was high expressed in e . coli after iptg induction . by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained . based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family 2.gdnf的表達(dá)及其對(duì)pc12基因工程細(xì)胞的影響用本教研室已構(gòu)建好的表達(dá)質(zhì)粒pet一gdnf轉(zhuǎn)化大腸桿菌bl21(de3),經(jīng)lmmipto誘導(dǎo)gdnf表達(dá),并在niz氣nta柱上進(jìn)行純化,稀釋復(fù)性后,純度達(dá)90%以上。