In this paper , one interactive protein was isolated and purified further by ion - exchange chromatography , whose molecular weight was determined by sds - page to be 24kda under non - reduced conditions and 25kda under reduced conditions 最后,分離純化作用蛋白中分子量為24kda的蛋白,發(fā)現(xiàn)其存在鏈內(nèi)二硫鍵,二硫鍵斷裂后的分子量為25kda 。
A man / glc - specific dolichos puepureus lectin ( dpl ) was purified from seeds of dolichos puepureus l . var . by a procedure including precipitated by ammonium sulphate , deae - 52 iron - exchange and sephacryl s - 300 hr gel filtration . the purified dpl showed a single band on page and ief gel , two bands on sds - page under reducing and non - reducing congditions 黑皮扁豆( dolichospurpureusl . var . )經(jīng)粉碎、浸取、硫酸銨分段鹽析、 deae - 52離子交換和sephacryls - 300hr分子篩層析可得到一表觀分子量為155kd的甘露糖葡萄糖專一的黑皮扁豆凝集素( dolichospurpureuslectin , dpl ) ,無血型專一性。